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cell culture human renal proximal tubule cells  (ATCC)


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    ATCC cell culture human renal proximal tubule cells
    Cell Culture Human Renal Proximal Tubule Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+renal+proximal+tubule+cells/HK-2/pm41963313-120-3-11
    Average 99 stars, based on 4520 article reviews
    cell culture human renal proximal tubule cells - by Bioz Stars, 2026-09
    99/100 stars

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    Cell Culture:

    Article Title: VHL-recruiting PROTAC attenuates AKI-CKD transition via simultaneous degradation of Smad3 and stabilization of HIF-2α.
    Article Snippet: Serum biochemical data of creatinine and BUN were analyzed through automatic method and equipment (Hitachi 717 Chemistry Analyzer, Tokyo, Japan). .. Cell lines and cell culture Human renal proximal tubule cells (HK2, ATCC, RRID:CVCL_0302), mCCDcl1 (CTCC001-0852, RRID:CVCL_B7HR) was cultured at 37 °C in 5% CO2 in mCCDcl1 medium (CTCC-001-085-CM, MeisenCTCC). ..



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    (a) Schematic and brightfield image of <t>RPTECs</t> at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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    ATCC cell culture human renal proximal tubule cells hk 2
    (a) Schematic and brightfield image of <t>RPTECs</t> at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
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    ATCC human renal proximal tubular epithelial cells culture human renal proximal tubular epithelial cells hrptecs
    (a) Schematic and brightfield image of <t>RPTECs</t> at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
    Human Renal Proximal Tubular Epithelial Cells Culture Human Renal Proximal Tubular Epithelial Cells Hrptecs, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC cell culture human renal proximal tubule epithelial cells
    (a) Schematic and brightfield image of <t>RPTECs</t> at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.
    Cell Culture Human Renal Proximal Tubule Epithelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+culture+human+renal+proximal+tubule+cells/Primary+Renal+Proximal+Tubule+Epithelial+Cells%3B+Normal%2C+Human/pmc06735734-67-0-15
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    Image Search Results


    (a) Schematic and brightfield image of RPTECs at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

    Journal: Biomaterials

    Article Title: Extended longevity geometrically-inverted proximal tubule organoids

    doi: 10.1016/j.biomaterials.2022.121828

    Figure Lengend Snippet: (a) Schematic and brightfield image of RPTECs at time of seeding, where cells are homogeneously mixed among partially gelled Matrigel. (b) Schematic and brightfield image of organoid formation within 24–48 hours. Scalebars represent 500 μm. (c) Time course brightfield of whole organoids, with the same organoid tracked over time. Scalebars represent 200 μm. (d) H&E images of representative organoid sections at days 8, 16, 30, and 90 of their growth. Scalebars represent 200 μm. (e) Diameter and (f) roundness values for the particular organoid shown in panel (c). (g) Area, diameter, and (h) roundness values for organoids for days 4–67 of their growth. (i) Quantification of cell coverage per circumference length of RPTEC organoids at days 8, 16, 30, and 90 of their growth. For panels (g) and (h), n = 16 per timepoint. For panel (i), n=5 per timepoint. One-way ANOVA was performed using Tukey’s multiple comparisons. ** denotes p ≤ 0.01 and **** denotes p ≤ 0.0001.

    Article Snippet: 2D culture Human renal proximal tubule epithelial cells (RPTECs) were grown in renal epithelial cell basal media (ATCC) supplemented with 0.5% fetal bovine serum (FBS), 10 nM triiodothyronine, 10 ng/mL epidermal growth factor (EGF), 100 ng/mL hydrocortisone hemisuccinate, 5 ug/mL insulin, 1.0 μM epinephrine, 5 μg/mL transferrin, 2.4 mM L-glutamine, and 1% penicillin-streptomycin (pen-strep).

    Techniques:

    (a) Volcano plot from bulk RNAseq comparing day 31 organoids with 2D RPTECs, and (b) day 67 with day 31 organoids. (c) Heatmap from bulk RNAseq comparing day 67 organoids, day 31 organoids, and 2D RPTECs. The colors in the heatmaps represent Z-scores computed from normalized gene counts. Normalized counts of genes of day 67 organoids, day 31 organoids, and 2D RPTECs for (d) AQP1, (e) LRP2, (f) SLC47A1, (g) CDH16, (h) TWIST2, and (i) MKI67. n = 3 for both 2D and organoid samples. These genes were significantly different based on the LRT with the Benjamini-Hochberg correction. **** denotes p ≤ 0.0001.

    Journal: Biomaterials

    Article Title: Extended longevity geometrically-inverted proximal tubule organoids

    doi: 10.1016/j.biomaterials.2022.121828

    Figure Lengend Snippet: (a) Volcano plot from bulk RNAseq comparing day 31 organoids with 2D RPTECs, and (b) day 67 with day 31 organoids. (c) Heatmap from bulk RNAseq comparing day 67 organoids, day 31 organoids, and 2D RPTECs. The colors in the heatmaps represent Z-scores computed from normalized gene counts. Normalized counts of genes of day 67 organoids, day 31 organoids, and 2D RPTECs for (d) AQP1, (e) LRP2, (f) SLC47A1, (g) CDH16, (h) TWIST2, and (i) MKI67. n = 3 for both 2D and organoid samples. These genes were significantly different based on the LRT with the Benjamini-Hochberg correction. **** denotes p ≤ 0.0001.

    Article Snippet: 2D culture Human renal proximal tubule epithelial cells (RPTECs) were grown in renal epithelial cell basal media (ATCC) supplemented with 0.5% fetal bovine serum (FBS), 10 nM triiodothyronine, 10 ng/mL epidermal growth factor (EGF), 100 ng/mL hydrocortisone hemisuccinate, 5 ug/mL insulin, 1.0 μM epinephrine, 5 μg/mL transferrin, 2.4 mM L-glutamine, and 1% penicillin-streptomycin (pen-strep).

    Techniques: